TasRail has cut 14 jobs after several mining operations in Tasmania failed to get off the ground.
The government-owned company said nine train drivers and five rail workers would be let go.
More than twenty people have been sacked since the start of the year.
TasRail was forced to make adjustments after Mt Lyell Copper and Nelson Bay River iron ore mine both stopped mining earlier this year.
ABC reports it had also banked on servicing the mothballed Riley Creek mine.
The Rail, Tram and Bus Union said the job cuts would lead to a loss of skilled workers in the state.